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  1. Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner c...

    Authors: Taras Pasternak, Olaf Tietz, Katja Rapp, Maura Begheldo, Roland Nitschke, Benedetto Ruperti and Klaus Palme
    Citation: Plant Methods 2015 11:50
    Content type: Methodology Published on:

  2. Since the quality and yield of rice production depends on endosperm development, previous studies have focused on the molecular mechanism that regulates this developmental process. Recently, how this process i...

    Authors: Quan Kuang, Xiaobo Yu, Xiongbo Peng and Meng-xiang Sun
    Citation: Plant Methods 2015 11:49
    Content type: Methodology Published on:

  3. Photosynthetic assimilation of carbon is a defining feature of the plant kingdom. The fixation of large amounts of carbon dioxide supports the synthesis of carbohydrates, which make up the bulk of plant biomas...

    Authors: Katharina Kölling, Gavin M. George, Roland Künzli, Patrick Flütsch and Samuel C. Zeeman
    Citation: Plant Methods 2015 11:48
    Content type: Methodology Published on:

  7. Plant growth chambers provide a controlled environment to analyse the effects of environmental parameters (light, temperature, atmospheric gas composition etc.) on plant function. However, it has been shown th...

    Authors: Amanda S. Porter, Christiana Evans-Fitz.Gerald, Jennifer C. McElwain, Charilaos Yiotis and Caroline Elliott-Kingston
    Citation: Plant Methods 2015 11:44
    Content type: Research Published on:

  8. The importance of appropriate, accurate measurement and reporting of environmental parameters in plant sciences is a significant aspect of quality assurance for all researchers and their research. There is a c...

    Authors: A J Both, L Benjamin, J Franklin, G Holroyd, L D Incoll, M G Lefsrud and G Pitkin
    Citation: Plant Methods 2015 11:43
    Content type: Commentary Published on:

  9. Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to th...

    Authors: Jason S. Cumbie, Sergei A. Filichkin and Molly Megraw
    Citation: Plant Methods 2015 11:42
    Content type: Methodology Published on:

  10. X-ray computed tomography (CT) has become a powerful tool for root phenotyping. Compared to rather classical, destructive methods, CT encompasses various advantages. In pot experiments the growth and developme...

    Authors: Johannes Pfeifer, Norbert Kirchgessner, Tino Colombi and Achim Walter
    Citation: Plant Methods 2015 11:41
    Content type: Methodology Published on:

  12. Bread wheat (Triticum aestivum L., 2n = 6x = 42) is an allohexaploid with a huge genome. Due to the presence of extensive homoeologs and paralogs, generating locus-specific sequences can be challenging, especiall...

    Authors: Jian Ma, Jiri Stiller, Zhi Zheng, Ya-Xi Liu, Yuming Wei, You-Liang Zheng and Chunji Liu
    Citation: Plant Methods 2015 11:39
    Content type: Methodology Published on:

  13. Higher plants have evolved sophisticated communication systems to integrate environmental stimuli into their developmental programs. Grafting provides a powerful technique to examine transportation and systemi...

    Authors: Nien-Chen Huang and Tien-Shin Yu
    Citation: Plant Methods 2015 11:38
    Content type: Methodology Published on:

  16. Recent developments in unmanned aerial platforms (UAP) have provided research opportunities in assessing land allocation and crop physiological traits, including response to abiotic and biotic stresses. UAP-ba...

    Authors: M Zaman-Allah, O Vergara, J L Araus, A Tarekegne, C Magorokosho, P J Zarco-Tejada, A Hornero, A Hernández Albà, B Das, P Craufurd, M Olsen, B M Prasanna and J Cairns
    Citation: Plant Methods 2015 11:35
    Content type: Methodology Published on:

  17. The genus Cuscuta is a group of parasitic plants that are distributed world-wide. The process of parasitization starts with a Cuscuta plant coiling around the host stem. The parasite’s haustorial organs then esta...

    Authors: Daisuke Ikeue, Christian Schudoma, Wenna Zhang, Yoshiyuki Ogata, Tomoaki Sakamoto, Tetsuya Kurata, Takeshi Furuhashi, Friedrich Kragler and Koh Aoki
    Citation: Plant Methods 2015 11:34
    Content type: Methodology Published on:

  18. A well characterized output of the circadian clock in plants is the daily rhythmic movement of leaves. This process has been used extensively in Arabidopsis to estimate circadian period in natural accessions a...

    Authors: Kathleen Greenham, Ping Lou, Sara E Remsen, Hany Farid and C Robertson McClung
    Citation: Plant Methods 2015 11:33
    Content type: Methodology Published on:

  19. Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With ...

    Authors: Andrew J Tindall, Jade Waller, Mark Greenwood, Peter D Gould, James Hartwell and Anthony Hall
    Citation: Plant Methods 2015 11:32
    Content type: Review Published on:

  20. The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50–100.000 ho...

    Authors: Martin Janda, Oldřich Navrátil, Daniel Haisel, Barbora Jindřichová, Jan Fousek, Lenka Burketová, Noemi Čeřovská and Tomáš Moravec
    Citation: Plant Methods 2015 11:31
    Content type: Methodology Published on:

  21. Genetic markers are tools that can facilitate molecular breeding, even in species lacking genomic resources. An important class of genetic markers is those based on orthologous genes, because they can guide hy...

    Authors: Jin-Hyun Kim, Chaeyoung Lee, Daejin Hyung, Ye-Jin Jo, Joo-Seok Park, Douglas R Cook and Hong-Kyu Choi
    Citation: Plant Methods 2015 11:30
    Content type: Methodology Published on:

  22. Current methods of in-house plant phenotyping are providing a powerful new tool for plant biology studies. The self-constructed and commercial platforms established in the last few years, employ non-destructiv...

    Authors: Jan F Humplík, Dušan Lazár, Alexandra Husičková and Lukáš Spíchal
    Citation: Plant Methods 2015 11:29
    Content type: Review Published on:

  23. The detection and characterization of resistance reactions of crop plants against fungal pathogens are essential to select resistant genotypes. In breeding practice phenotyping of plant genotypes is realized b...

    Authors: Matheus Kuska, Mirwaes Wahabzada, Marlene Leucker, Heinz-Wilhelm Dehne, Kristian Kersting, Erich-Christian Oerke, Ulrike Steiner and Anne-Katrin Mahlein
    Citation: Plant Methods 2015 11:28
    Content type: Methodology Published on:

  25. Grass stalks architecturally support leaves and reproductive structures, functionally support the transport of water and nutrients, and are harvested for multiple agricultural uses. Research on these basic and...

    Authors: Sven Heckwolf, Marlies Heckwolf, Shawn M Kaeppler, Natalia de Leon and Edgar P Spalding
    Citation: Plant Methods 2015 11:26
    Content type: Methodology Published on:

  27. Image analysis is increasingly used in plant phenotyping. Among the various imaging techniques that can be used in plant phenotyping, chlorophyll fluorescence imaging allows imaging of the impact of biotic or ...

    Authors: Céline Rousseau, Gilles Hunault, Sylvain Gaillard, Julie Bourbeillon, Gregory Montiel, Philippe Simier, Claire Campion, Marie-Agnès Jacques, Etienne Belin and Tristan Boureau
    Citation: Plant Methods 2015 11:24
    Content type: Methodology Published on:

  28. Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a p...

    Authors: Justine Bresson, François Vasseur, Myriam Dauzat, Garance Koch, Christine Granier and Denis Vile
    Citation: Plant Methods 2015 11:23
    Content type: Methodology Published on:

  31. Recently emerging approaches to high-throughput plant phenotyping have discovered their importance as tools in unravelling the complex questions of plant growth, development and response to the environment, bo...

    Authors: Jan F Humplík, Dušan Lazár, Tomáš Fürst, Alexandra Husičková, Miroslav Hýbl and Lukáš Spíchal
    Citation: Plant Methods 2015 11:20
    Content type: Methodology Published on:

  32. Detection of induced mutations is valuable for inferring gene function and for developing novel germplasm for crop improvement. Many reverse genetics approaches have been developed to identify mutations in gen...

    Authors: Leonardo Galindo-González, David Pinzón-Latorre, Erik A Bergen, Dustin C Jensen and Michael K Deyholos
    Citation: Plant Methods 2015 11:19
    Content type: Methodology Published on:

  33. Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transpo...

    Authors: Alexander K Converse, Elizabeth O Ahlers, Tom W Bryan, Jackson D Hetue, Katherine A Lake, Paul A Ellison, Jonathan W Engle, Todd E Barnhart, Robert J Nickles, Paul H Williams and Onofre T DeJesus
    Citation: Plant Methods 2015 11:18
    Content type: Research Published on:

  34. Roots are vital to plants for soil exploration and uptake of water and nutrients. Root performance is critical for growth and yield of plants, in particular when resources are limited. Since roots develop in s...

    Authors: Ralf Metzner, Anja Eggert, Dagmar van Dusschoten, Daniel Pflugfelder, Stefan Gerth, Ulrich Schurr, Norman Uhlmann and Siegfried Jahnke
    Citation: Plant Methods 2015 11:17
    Content type: Methodology Published on:

  35. Biological fluids often have interesting and unusual physical properties to adapt them for their specific purpose. Laboratory-based rheometers can be used to characterise the viscoelastic properties of such fl...

    Authors: Catherine Collett, Alia Ardron, Ulrike Bauer, Gary Chapman, Elodie Chaudan, Bart Hallmark, Lee Pratt, Maria Dolores Torres-Perez and D Ian Wilson
    Citation: Plant Methods 2015 11:16
    Content type: Methodology Published on:

  36. Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that ter...

    Authors: Ralf Gente and Martin Koch
    Citation: Plant Methods 2015 11:15
    Content type: Review Published on:

  37. Plant phenotyping refers to a quantitative description of the plant’s anatomical, ontogenetical, physiological and biochemical properties. Today, rapid developments are taking place in the field of non-destruc...

    Authors: Achim Walter, Frank Liebisch and Andreas Hund
    Citation: Plant Methods 2015 11:14
    Content type: Review Published on:

  38. Water availability is a major limiting factor for wheat (Triticum aestivum L.) production in rain-fed agricultural systems worldwide. Root system architecture has important functional implications for the timing ...

    Authors: Cecile AI Richard, Lee T Hickey, Susan Fletcher, Raeleen Jennings, Karine Chenu and Jack T Christopher
    Citation: Plant Methods 2015 11:13
    Content type: Methodology Published on:

  40. Three-dimensional canopies form complex architectures with temporally and spatially changing leaf orientations. Variations in canopy structure are linked to canopy function and they occur within the scope of g...

    Authors: Mark Müller-Linow, Francisco Pinto-Espinosa, Hanno Scharr and Uwe Rascher
    Citation: Plant Methods 2015 11:11
    Content type: Software Published on:

  41. Plant phenotype datasets include many different types of data, formats, and terms from specialized vocabularies. Because these datasets were designed for different audiences, they frequently contain language a...

    Authors: Anika Oellrich, Ramona L Walls, Ethalinda KS Cannon, Steven B Cannon, Laurel Cooper, Jack Gardiner, Georgios V Gkoutos, Lisa Harper, Mingze He, Robert Hoehndorf, Pankaj Jaiswal, Scott R Kalberer, John P Lloyd, David Meinke, Naama Menda, Laura Moore…
    Citation: Plant Methods 2015 11:10
    Content type: Methodology Published on:

  42. Field-based high throughput phenotyping is a bottleneck for crop breeding research. We present a novel method for repeated remote phenotyping of maize genotypes using the Zeppelin NT aircraft as an experimenta...

    Authors: Frank Liebisch, Norbert Kirchgessner, David Schneider, Achim Walter and Andreas Hund
    Citation: Plant Methods 2015 11:9
    Content type: Methodology Published on:

  43. The water status in plant leaves is a good indicator for the water status in the whole plant revealing stress if the water supply is reduced. The analysis of dynamic aspects of water availability in plant tiss...

    Authors: Said Dadshani, Andriy Kurakin, Shukhrat Amanov, Benedikt Hein, Heinz Rongen, Steve Cranstone, Ulrich Blievernicht, Elmar Menzel, Jens Léon, Norbert Klein and Agim Ballvora
    Citation: Plant Methods 2015 11:8
    Content type: Methodology Published on:

  45. We review a set of recent multiscale imaging techniques, producing high-resolution images of interest for plant sciences. These techniques are promising because they match the multiscale structure of plants. H...

    Authors: David Rousseau, Yann Chéné, Etienne Belin, Georges Semaan, Ghassen Trigui, Karima Boudehri, Florence Franconi and François Chapeau-Blondeau
    Citation: Plant Methods 2015 11:6
    Content type: Review Published on:

  46. The dietary carotenoids serve as precursor for vitamin A and prevent several chronic-degenerative diseases. The carotenoid profiling is necessary to understand their importance on human health. However, the av...

    Authors: Prateek Gupta, Yellamaraju Sreelakshmi and Rameshwar Sharma
    Citation: Plant Methods 2015 11:5
    Content type: Methodology Published on:

  47. Piercing-sucking insects are major vectors of plant viruses causing significant yield losses in crops. Functional genomics of plant resistance to these insects would greatly benefit from the availability of hi...

    Authors: Karen J Kloth, Cindy JM ten Broeke, Manus PM Thoen, Marianne Hanhart-van den Brink, Gerrie L Wiegers, Olga E Krips, Lucas PJJ Noldus, Marcel Dicke and Maarten A Jongsma
    Citation: Plant Methods 2015 11:4
    Content type: Methodology Published on:

  48. Well-developed and functional roots are critical to support plant life and reach high crop yields. Their study however, is hampered by their underground growth and characterizing complex root system architectu...

    Authors: Laura Mathieu, Guillaume Lobet, Pierre Tocquin and Claire Périlleux
    Citation: Plant Methods 2015 11:3
    Content type: Methodology Published on:

  49. During the last fifteen years, cell wall proteomics has become a major research field with the publication of more than 50 articles describing plant cell wall proteomes. The WallProtDB database has been designed ...

    Authors: Hélène San Clemente and Elisabeth Jamet
    Citation: Plant Methods 2015 11:2
    Content type: Database Published on:

  50. Combined assessment of leaf reflectance and transmittance is currently limited to spot (point) measurements. This study introduces a tailor-made hyperspectral absorption-reflectance-transmittance imaging (Hype...

    Authors: Sergej Bergsträsser, Dimitrios Fanourakis, Simone Schmittgen, Maria Pilar Cendrero-Mateo, Marcus Jansen, Hanno Scharr and Uwe Rascher
    Citation: Plant Methods 2015 11:1
    Content type: Methodology Published on:
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ISSN: 1746-4811