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Fig. 7 | Plant Methods

Fig. 7

From: Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR

Fig. 7

A MPCR amplification of chromosomes 1–7 of the A, B, and D sub-genome of wheat and the H genome of barley using various wheat and barley species. B Improved MPCR amplification with substituted primer pairs specific for the 6 A and 7B chromosomes. M – Molecular size marker (GeneRuler™ 100 bp Plus DNA Ladder). Reaction components: Phusion Green HF Buffer and Phusion Hot Start II High-Fidelity DNA Polymerase

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Plant Methods

ISSN: 1746-4811