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Table 2 Optimized conditions of the cotyledon-VIGS in two medicinal plants

From: A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants

Plants/Conditions

Glycyrrhiza

inflata

Artemisia

annua

Seedling age

(days)

7

6

Infiltration time

(minutes)

60

10

OD600 of

infiltration solution

1.0

0.5

Efficiency*

30/30

50/50

  1. *After infiltration with the VIGS vectors, the seedlings were incubated for 6 days (3 days in dark and then 3 days in 16 h light /8 h dark regime), to record yellow cotyledon phenotype. The efficiency is shown as the number of yellow cotyledons per 30–50 cotyledons
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Plant Methods

ISSN: 1746-4811