Fig. 5

Cotyledon-VIGS of CrGBF1/GBF2 and overexpression of CrMYC2. (a) Relative expression of CrGBF1, CrGBF2, CrMYC2, TDC and STR in empty vector control (TRV) and CrGBF1/GBF2/MYC2 cotyledons. (b) Relative expression of CrMYC2, TDC and STR in empty vector control and CrMYC2 overexpression cotyledons. Relative expression was measured by RT-qPCR, and the C. roseus RPS9 gene was used as an internal reference gene. The values represent means ± SD from three biological replicates. For each biological replicate, entire cotyledons were pooled from 8–9 seedlings (16–17 cotyledons). Statistical significance was calculated using Student’s t test (** P < 0.01)