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Fig. 5 | Plant Methods

Fig. 5

From: Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)

Fig. 5

Silencing of miR396 in O. javanica using the CMV-based VbMS vector. A, Schematic representation of VbMS of miR396. B, Phenotypes of plants inoculated with CMV-STTM396 at 40 dpi. C, Systematic leaf morphology analysis of pCB301CMV-STTM396 in ‘Fq1’ (40 dpi). Scale bars are 2 cm. D, RT‒PCR detection confirmed the expression of STTM396 in CMV-STTM396-infected plants. E, Stem‒loop RT‒qPCR analysis of miR396 expression levels in CMVâ–³2b- or CMV-STTM396-infected plants. F, Detection of the relative expression level of the miR396 target gene Growth-Regulating Factor 9-like

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Plant Methods

ISSN: 1746-4811