Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)

Fig. 5

Relative expression level of various genes in different sample sets using the most and the least stable reference genes for normalization. a, b Relative expression levels of AsPKP1 (a) and AsAGPL2 (b) in developing seeds. EP+EF1A represents the most stable RG set and GAPDH1 is the least stable RG. Error bars indicate SD of three replicates. The B, C, D, E, G, I and J refer to developing stages of oat seeds [7]. c, d Relative expression levels of AsPKP1 (c) and AsAGPL2 (d) in developing endosperms. EIF4A+HNR represents the most stable RG set and GAPDH1 is the least stable RG. Error bars indicate SD of three replicates. The B, C, D, E, G, I and J refer to developing stages of oat seeds [7]. e, f Relative expression levels of AsSGT1 (e) and AsSCL14 (f) in shoots and roots of seedlings at two-leaf and three-leaf stage. UBC21+HNR represents the most stable RG set and 18S is the least stable RG. Error bars indicate SD of three replicates

Back to article page

Plant Methods

ISSN: 1746-4811