Fig. 6
From: Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana
Effects of co‐expressing virus‐derived genes encoding cell cycle regulatory proteins. Agrobacteria strain AGL1 harbouring pEAQ‐GSN were co‐infiltrated into N. benthamiana leaves with a TYDV Rep/RepA or RepA genes and the TYDV RepA gene containing a LxCxK mutation in the RB-binding motif, and b cell cycle regulatory genes from related circular ssDNA plant viruses, under the transcriptional control of the truncated CaMV 35S (− 90) promoter (Δ35S). Leaves were sampled 4 dpi and TSP extracted for GUS fluorometric enzyme assays. Columns represent relative levels of mean GUS enzyme activities and bars represent ± SE. (†) indicates the reference treatment and (*) indicates data significantly different to the reference (p < 0.05)