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Fig. 5 | Plant Methods

Fig. 5

From: Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana

Fig. 5

Effects of co‐expressing suppressors of gene silencing on transient GUS expression. Agrobacteria strain AGL1 harbouring the vector p35S‐GSN were co‐infiltrated into N. benthamiana leaves with various suppressors of gene silencing, including CMV 2b, CMV 2b (1-94) truncation, PRSV HC-Pro, TBSV p19, TLCV TrAP and both the CMV 2b (1-94) truncation and TBSV p19 together. Leaves were sampled 4 dpi and TSP extracted for GUS fluorometric enzyme assays. Columns represent relative levels of mean GUS enzyme activities and bars represent ± SE. (†) indicates the reference treatment and (*) indicates data significantly different to the reference (p < 0.05)

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