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Fig. 2 | Plant Methods

Fig. 2

From: Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana

Fig. 2

Effects of agrobacteria strain and cell culture density on transient GUS expression via agroinfiltration. a Agrobacteria strains AGL1, C58C1 and LBA4404 harbouring pEAQ‐GSN were infiltrated into N. benthamiana leaves. Leaves were sampled at 0, 2, 4, 6 and 8 days post infiltration (dpi). b Agrobacterium strain AGL1 harbouring pEAQ‐GSN was infiltrated into N. benthamiana leaves at increasing concentrations OD600 = 0.001, 0.01, 0.1, 0.5, 1.0, and 1.5. TSP was extracted for GUS fluorometric enzyme assays. Columns represent relative levels of mean GUS enzyme activities and bars represent ± SE. (†) indicates the reference treatment and (*) indicates data significantly different to the reference (p < 0.05)

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