Parameter tested | Levels | Transformation efficiency | Statistics |
---|---|---|---|
Sugar content in the medium | 0% | 2.26 ± 0.8c | Pr > F < 0.0001 |
0.5% | 11.74 ± 5.0bc | ||
1% | 20.84 ± 5.3b | ||
3% | 73.93 ± 9.0a | ||
4% | 74.07 ± 6.0a | ||
Age of the seedling | 5 days | 67.91 ± 4.1a | Pr > F < 0.0013 |
10 days | 54.18 ± 2.4b | ||
15 days | 37.31 ± 8.1c | ||
Acetosyringone | 0 | 3.25 ± 0.9c | Pr > F < 0.0001 |
200 uM | 21.74 ± 4.5b | n = 366–376 | |
400 uM | 65.03 ± 6.4a |  | |
Days kept in the co-incubation plate | 7 days | 33.21 ± 8.5c | Pr > F < 0.0008 |
14 days | 68.33 ± 4.2a | ||
21 days | 61.26 ± 4.1a | ||
Explant type | Hypocotyl | 67.20 ± 2.8a | Pr > |t| < 0.0001 |
Root tip | 0.76 ± 0.8b | ||
Illumination during co-incubation | Light | 67.53 ± 6.7a | Pr > |t| < 0.0001 |
Dark | 12.46 ± 2.6b |