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Table 3 Average relative standard deviations of individual amino acid concentrations in a sample injected 10 times (injection repeatability), in 5 derivatizations from the same hydrolysate (derivatization repeatability), or in 12 different matrices analyzed three times each day for 3 days

From: High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry

Amino acid

Injection repeatability (%) (n = 10)

Derivatization repeatability (%) (n = 5)

Average reproducibility (%)

Alanine

1.4

3.0

3.8

Arginine

2.3

1.8

4.9

Glycine

1.1

0.8

3.9

Histidine

2.2

2.7

7.7

Hydroxyproline

3.3

4.0

18

Isoleucine

0.8

1.4

4.1

Leucine

1.0

2.9

4.6

Lysine

3.7

3.2

5.3

Phenylalanine

0.9

2.4

5.5

Proline

0.8

4.2

5.6

Serine

1.2

0.7

4.0

Threonine

1.1

1.7

4.1

Tyrosine

1.1

5.5

5.1

Valine

0.9

2.9

4.5

Aspartic acid

1.1

2.2

3.6

Glutamic acid

0.9

2.9

4.0

Cysteic acid

N.D.

N.D.

6.0

Methionine sulfone

N.D.

N.D.

5.7

  1. N.D. not determined