Table 2 List of the primers and TaqMan probes designed in this study
Target epitope/gene | Oligonucleotide | Sequence |
|---|---|---|
Epitopes | ||
DQ2.5-glia-α1 | Forward primer | 5′-GCAACCATTTCCATCACAACWAC-3′ |
Reverse primer | 5′-GTGSTTGCGAATACTGTGGTTG-3′ | |
Probe | 5′-FAM-WTCCRCAGCCGCAACTACCA-TAMRA-3′ | |
DQ2.5-glia-α2 | Forward primer | 5′-GCAACCATTTCCATCACAACWAC-3′ |
Reverse primer | 5′-GTGSTTGCGAATACTGTGGTTG-3′ | |
Probe | 5′-FAM-AGCCGCAACTACCATATCCGC-TAMRA-3′ | |
DQ2.5-glia-α3 | Forward primer | 5′-GCAACCATTTCCATCACAACWAC-3′ |
Reverse primer | 5′-GTGSTTGCGAATACTGTGGTTG-3′ | |
Probe | 5′-FAM-TTCGACCACAACAACCATATCCAC-TAMRA-3′ | |
DQ8-glia-α1 | Forward primer | 5′-CCACAATGTYGTTCATGCTATTATTCTGC-3′ |
Reverse primer | 5′-CAGAGCCCTGGGCCTGTGG-3′ | |
Probe | 5′-FAM-AGGGCTCCTTCCAGCCAT-TAMRA-3′ | |
Reference genes | ||
ADP-ribosylation factor (ARF) | Forward primer | 5′-GCTCTCCAACAACATTGCCAAC-3′ |
Reverse primer | 5′-GCTTCTGCCTGTCACATACGC-3′ | |
Probe | 5′-FAM-CAAGAAACAAACGTGCTGGATGTC-TAMRA-3′ | |
Similar to RNase L inhibitor-like protein (RLI) | Forward primer | 5′-CGATTCAGAGCAGCGTATTGTTGC-3′ |
Reverse primer | 5′-AGTTGGTCGGGTCTCTTCTAAATGTAATG-3′ | |
Probe | 5′-FAM-CTTAGCGGACAAGGTTATTGTTTATGAGG-TAMRA-3′ | |
Vacuolar ATP synthase 16 kDa proteolipid sub. (VAS) | Forward primer | 5′-GCTGGAGTGCTCGGTATCTACGG-3′ |
Reverse primer | 5′-TGCGAAGATGAGGATGAGGATCA-3′ | |
Probe | 5′-FAM-ATCGGCATTGTTGGTGATGCT-TAMRA-3′ | |
Ubiquinol-cytochrome C reduct. iron-sulfur sub. (UCC) | Forward primer | 5′-CCTGCCCCGTACAACCTTGAG-3′ |
Reverse primer | 5′-TCACCGTTGCGATAGTCCTGAAAC-3′ | |
Probe | 5′-FAM-ACAGGAGTGAATTCCTGTTGCGC-TAMRA-3′ | |
GABA-receptor-associated protein (GABA) | Forward primer | 5′-TTACGAGGAGAACAAGGACGAGGA-3′ |
Reverse primer | 5′-CAGGAGGCATTCAGAGCGATTG-3′ | |
Probe | 5′-FAM-CACCTTCGGATTGCTCTAGATGGC-TAMRA-3′ | |
Protein of unknown function [DUF52 family] (DUF52) | Forward primer | 5′-TGGTGCCATTCACAAATCAATCG-3′ |
Reverse primer | 5′-GCGAACAAACCCGACCTTAATCTTC-3′ | |
Probe | 5′-FAM-CATGGAGATCATAGAGACTGGTGACC-TAMRA-3′ | |
Cell division control protein (CDC) | Forward primer | 5′-CAAATACGCCATCAGGGAGAACATC-3′ |
Reverse primer | 5′-CGCTGCCGAAACCACGAGAC-3′ | |
Protein transport protein Sec23A (SEC23) | Forward primer | 5′-AGCAATTCGCACAATTATTACAAGCTC-3′ |
Reverse primer | 5′-GATGCTCACAGAAGACCTGGAAGC-3′ | |
Superoxide dismutase [Cu–Zn] (SOD) | Forward primer | 5′-CCTTACTGGACCAAATTCAATTGTTGG-3′ |
Reverse primer | 5′-GGTGCACACTAACAAGTGATCAAAGATC-3′ | |
S-adenosylmethionine decarboxylase (SAD) | Forward primer | 5′-GGCTGGACAAGAAGAAGGCCTCT-3′ |
Reverse primer | 5′-ATGGATGGTGGAGACGGCAGAT-3′ | |