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Figure 2 | Plant Methods

Figure 2

From: Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

Figure 2

Tape- Arabidopsis Sandwich method generates sufficient protoplasts for transient transfection and leaves of any stage can be used. A. Different weights of leaves (25, 50, 100, 150 mg) were incubated in 2 ml digestion buffer (row 1), protoplasts were collected (row 2) and 1.20 × 104, 3.15 × 104, 6.84 × 104, and 5.49 × 105 protoplasts were obtained (row 3). Bar: row 1 and 2 = 0.5 cm, row 3 = 0.5 mm. B. Arabidopsis leaves incubated in digestion buffer for 0, 5, 10, 15, 20 min. The protoplasts were fully released within 30 min. Bar = 1 cm. C. Both mature leaf (left) and two week-old seedlings (middle and right) could be used for protoplast isolation. Bar = 1 cm.

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