Table 1 Oligonucleotides used to generate epitope tags. Restriction sites are in bold and linker sequences are underlined.
From: A modified Gateway cloning strategy for overexpressing tagged proteins in plants
Oligo name | Sequence 5'-3' |
|---|---|
nSTREPfw | TCGAC ATGTGGAGCCATCCGCAGTTCGAAAAAGGCGGCAGCGGCG |
nSTREPrev | GATCC GCCGCTGCCGCC TTTTTCGAACTGCGGATGGCTCCACATG |
nHAfw | GAAGTCGAC ATGTCGCGATACCCCTAC |
nHArev | GAAGGATCC TCCACTGCTAGC GGCGTAG |
nMycfw | GAGTCGAC GGTATCGATTTAAAGC |
nMycrev | GAAGGATCC CGGGCTTCCGGA ATTCAAGTCCTCTTC |
nGSTfw | GAAGTCGAC ATGTCCCCTATACTAGGTTATTG |
nGSTrev | ACGGGATCC ACGCGGAACCAG ATC |
nYFPfw | GGGTCGAC ATGGTGAGCAAGGGCG |
nYFPrev | CCGGATCC AGGCTTGTACAGCTCGTCCATG |
cSTREPfw | GGCCGC AAGCGGCGGA TGGAGCCATCCGCAGTTCGAAAAATAGC |
cSTREPrev | TCGAG CTATTTTTCGAACTGCGGATGGCTCCATCCGCCGCTTGC |
cHAfw | AGCGGCCGC AAGCGGAGGCCTGTCGCGA TAC |
cHArev | GAACTCGAG AGTACTGCTCTAGGCTTAGTCGGGCAC |
cMycfw | GAAGCGGCCGC ACAAGCT ATGGAGCAAAAGC |
cMycrev | GAACTCGAG TCAGGAATTCAAGTCCTC |
cGSTfw | AGCGGCCGC AAGCGGAGGC ATGTCCCCTATACTAGG |
cGSTrev | GAACTCGAG CTAATCCGATTTTGGAGGATGG |
cYFPfw | GGGCGGCCGC ACCTATGGTGAGCAAGGGCG |
cYFPrev | CCCTCGAG TTACTTGTACAGCTCGTCCATG |