Figure 5
From: A modified Gateway cloning strategy for overexpressing tagged proteins in plants
Expression analysis of transformed Arabidopsis lines. Protein extracts (20 ÎĽg) from wild type (WT) and different independent Arabidopsis transgenic lines transformed with pH2n-H2A (A), pH2c-H2A (B), pH2n-UBI (C), pE5n-UBI (D) were separated on 15% SDS-PAGE and analysed by western blotting using antibodies against HA (A-C) or GFP (D). Ponceau staining is shown as loading control below each panel. E, Protein extracts from wild type (WT) and an Arabidopsis transgenic line expressing Strep-Ubiquitin were used for affinity purification on a Streptactin resin. Bound proteins were resolved on 15% SDS-PAGE and analysed by western blotting using an antibody against the STREP epitope. Ponceau staining at the bottom shows the presence of streptactin in both samples.