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Figure 5 | Plant Methods

Figure 5

From: Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells

Figure 5

Control experiments show specificity of multi-color BiFC experiments. Onion cells were transfected by particle bombardment and visualized using laser scanning confocal microscopy. Labels above each set of panels indicate the various constructions introduced into the cells. Labels below each set of panels indicates the filter set/channel imaged. mCherry-VirD2NLS labels the nucleus. In Panels A and B, respectively, VirE2-cCFP interacts with Impa-1-nVenus or Impa-4-nVenus expressed individually. Localization of yellow fluorescence is identical to that seen when both prey proteins are co-expressed with VirE2-cCFP. Note that in Panel C, yellow fluorescence is not reconstituted in the absence of interacting bait and prey proteins. DIC, differential interference contrast image. Size bars indicate 50 microns.

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