Figure 6

Transgenic RNA visualized in 3D interphase nuclei of root tissue sections from wheat (A), tobacco (B) and of an isolated tobacco nucleus (C). The wheat transgenic line illustrated in (A) carries five transgene copies at two sites on metaphase chromosomes as described in [2]. The tobacco transgenic line shown in B and C contains seven transgene copies. (D) Simultaneous localization of transgene loci and their transcript in a wheat endosperm nucleus 9 days after pollination. Nuclei counterstained with DAPI (blue) were hybridized with probes for the gene flanking regions and vector sequences of HMW-1Ax1 and HMW-1Dx5 to detect the locus (green, Alexa Fluor 488) and with an antisense probe for the 1Ax1 coding region to detect the transcripts (red, Alexa Fluor 633). The coding sequences of the two high molecular weight glutenin genes are highly homologous and cross-hybridize. The image is a projection of serial confocal sections. Section spacing, 0.6 μm. Bars, 10 μm.